Description
Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofN-MID-OTin the sample, This N-MID-OTELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusN-MID-OTconcentration. The concentration ofN-MID-OTinthe samples is then determined by comparing the O.D. of the samples to the standard curve